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KMID : 0370220110550010016
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Yakhak Hoeji
2011 Volume.55 No. 1 p.16 ~ p.21
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Development of a Quantitative ELISA for Anti HER-2 Antibodies using Human HER-2 Recombinant Proteins
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Jung Sun-Ki
Ryu Chang-Seon
Cheong Kyu-Jin
Song Gyu-Yong
Kim Sang-Kyum
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Abstract
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HER-2 (Human Epidermal Growth Factor Receptor-2) is a protein giving higher aggressiveness in human breast cancers. Trastuzumab is a monoclonal antibody that targets HER-2 and is known to extend survival across all stages of HER2-positive breast cancer. In this study, we attempted to development of a quantitative ELISA (Enzyme-Linked ImmunoSorbent Assay) for evaluating anti HER-2 antibodies using human HER-2 recombinant proteins to support antibody producing processes and pharmacokinetic studies. We established direct or indirect ELISA method for the trastuzumab-like protein combined human recombinant HER-2. The ELISA method will prove to be great value in quantitating anti-HER-2 antibodies levels for developing anticancer antibodies.
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KEYWORD
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ELISA, Trastuzumab, HER-2, breast cancer
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